Structural determination of the O-specific chain of the lipopolysaccharide from the mushrooms pathogenic bacterium Pseudomonas tolaasii

Antonio Molinaro; Emiliano Bedini; Renata Ferrara; Rosa Lanzetta; Michelangelo Parrilli; Antonio Evidente; Pietro Lo Cantore; Nicola Sante Iacobellis

doi:10.1016/s0008-6215(03)00112-5

Structural determination of the O-specific chain of the lipopolysaccharide from the mushrooms pathogenic bacterium Pseudomonas tolaasii

Carbohydr Res. 2003 May 23;338(11):1251-7. doi: 10.1016/s0008-6215(03)00112-5.

Authors

Antonio Molinaro¹, Emiliano Bedini, Renata Ferrara, Rosa Lanzetta, Michelangelo Parrilli, Antonio Evidente, Pietro Lo Cantore, Nicola Sante Iacobellis

Affiliation

¹ Dipartimento di Chimica Organica e Biochimica, Università di Napoli Federico II, Complesso Universitario Monte Sant' Angelo, Via Cintia 4, 80126, Napoli, Italy. molinaro@unina.it

PMID: 12747869
DOI: 10.1016/s0008-6215(03)00112-5

Abstract

The complete structure of the O-specific polysaccharide of the lipopolysaccharide isolated from the cultivated mushrooms pathogen Pseudomonas tolaasii is described. The structural determination, achieved by chemical and spectroscopical analyses, indicates a novel tetrasaccharide repeating unit built up of two units of 2-acetamido-2,6-di-deoxy-glucopyranose (Quinovosamine, QuipNAc) and two units of 2-acetamido-2-deoxy-gulopyranuronamide (GulpNAcAN), one of which is acetylated at C-3 position:

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Agaricales
Lipopolysaccharides / chemistry*
Lipopolysaccharides / isolation & purification
Nuclear Magnetic Resonance, Biomolecular / methods
O Antigens / chemistry*
O Antigens / isolation & purification
Pseudomonas / chemistry*

Substances

Lipopolysaccharides
O Antigens