Tumor-infiltrating T cells with similar antigen binding sites in different mucosa-associated lymphoid tissue type B cell lymphomas

Virchows Arch. 2003 Apr;442(4):343-8. doi: 10.1007/s00428-003-0758-6. Epub 2003 Feb 19.

Abstract

B cell lymphomas of mucosa-associated lymphoid tissue (MALT)-type arise on the background of chronic inflammation due to either autoimmunity or infection at various sites of the organism. Characteristically the tumor-infiltrating T cells found in large numbers in MALT-type lymphomas are predominantly of CD4(+) phenotype and may have impact on tumor pathogenesis. To assess whether the chromosomal translocation t(11;18)(q21; q21) that is specific for at least a subset of MALT-type lymphomas may have an impact on tumor environment, we investigated the antigen binding sites of tumor-infiltrating T cells from one t(11;18)-positive tumor of the thyroid and one t(11;18)-negative tumor of the stomach. MHC Allelotyping was performed and revealed common alleles in HLA-DR and HLA-DQ loci. Cloning and sequencing of the complementary determining region 3 in V(beta2) chains of T cell receptors demonstrated the use of identical J(beta)segments in both patients, suggestive for recognition of the same antigen. We therefore suggest that tumor-infiltrating CD4(+) T cells are tightly integrated in MALT-type lymphoma immunoarchitecture irrespective of genetic background and localization of the tumors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Binding Sites, Antibody / genetics
  • Binding Sites, Antibody / immunology
  • Chromosomes, Human, Pair 11
  • Chromosomes, Human, Pair 18
  • Clone Cells
  • Complementarity Determining Regions / genetics
  • Complementarity Determining Regions / immunology
  • DNA, Neoplasm / analysis
  • Flow Cytometry
  • HLA-DQ Antigens / genetics
  • HLA-DR Antigens / genetics
  • Humans
  • Immunoenzyme Techniques
  • Lymphocytes, Tumor-Infiltrating / immunology
  • Lymphocytes, Tumor-Infiltrating / pathology*
  • Lymphoma, B-Cell, Marginal Zone / genetics
  • Lymphoma, B-Cell, Marginal Zone / immunology
  • Lymphoma, B-Cell, Marginal Zone / pathology*
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • RNA, Neoplasm / analysis
  • Stomach Neoplasms / genetics
  • Stomach Neoplasms / immunology
  • Stomach Neoplasms / pathology
  • T-Lymphocytes, Helper-Inducer / immunology
  • T-Lymphocytes, Helper-Inducer / pathology*
  • Thyroid Neoplasms / genetics
  • Thyroid Neoplasms / immunology
  • Thyroid Neoplasms / pathology
  • Translocation, Genetic

Substances

  • Complementarity Determining Regions
  • DNA, Neoplasm
  • HLA-DQ Antigens
  • HLA-DR Antigens
  • RNA, Neoplasm