B cell complement receptor 2 transfer reaction

J Immunol. 2003 Apr 1;170(7):3671-8. doi: 10.4049/jimmunol.170.7.3671.

Abstract

The B cell C receptor specific for C3dg (CR2) shares a number of features with the primate E C receptor (CR1). Previously, we have demonstrated, both in vitro and in animal models, that immune complexes (IC) bound to primate E CR1, either via C opsonization or by means of bispecific mAb complexes, can be transferred to acceptor macrophages in a process that also removes CR1 from the E. We have now extended this paradigm, the transfer reaction, to include B cell CR2. We used both flow cytometry and fluorescence microscopy to demonstrate that IC bound to Raji cell CR2, either via C opsonization or through the use of an anti-CR2 mAb, are transferred to acceptor THP-1 cells. This reaction, which appears to require Fc recognition of IgG bound to Raji cell CR2, also leads to transfer of CR2. Additional support for the B cell transfer reaction is provided in a prototype study in a monkey model in which IC bound to B cell CR2 are localized to the spleen. These findings may have important implications with respect to defining the role of C in IC handling during the normal immune response.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Bispecific / metabolism
  • Antibodies, Monoclonal / metabolism
  • Antigen Presentation
  • Antigen-Antibody Complex / metabolism
  • B-Lymphocytes / immunology*
  • B-Lymphocytes / metabolism*
  • Cell Line, Transformed
  • Humans
  • Macaca fascicularis
  • Opsonin Proteins / metabolism
  • Protein Binding / immunology
  • Receptors, Complement 3b / metabolism
  • Receptors, Complement 3d / immunology
  • Receptors, Complement 3d / metabolism*
  • Rosette Formation
  • Tumor Cells, Cultured

Substances

  • Antibodies, Bispecific
  • Antibodies, Monoclonal
  • Antigen-Antibody Complex
  • Opsonin Proteins
  • Receptors, Complement 3b
  • Receptors, Complement 3d