The role of lipopolysaccharide in T-cell responses following DNA vaccination

Vaccine. 2003 Mar 28;21(13-14):1548-53. doi: 10.1016/s0264-410x(02)00676-x.

Abstract

Bacterial products, including lipopolysaccharide (LPS), are potential impurities in plasmid DNA vaccines. LPS has immunostimulatory properties even at exceedingly low concentrations through activation of Toll-like receptor 4 (TLR4). The potency of T-cell responses after vaccination was tested with DNA containing high LPS or depleted of LPS in TLR4-competent and TLR4-deficient mice. CD8(+) T-cell responses were readily induced in TLR4-deficient mice immunized with DNA depleted of LPS. LPS in DNA vaccines is not required for CD8(+) T-cell responses.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • CD8-Positive T-Lymphocytes / immunology*
  • Dendritic Cells / drug effects
  • Lipopolysaccharides / pharmacology*
  • Membrane Glycoproteins / physiology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Receptors, Cell Surface / physiology
  • Toll-Like Receptor 4
  • Toll-Like Receptors
  • Vaccination
  • Vaccines, DNA / immunology*

Substances

  • Lipopolysaccharides
  • Membrane Glycoproteins
  • Receptors, Cell Surface
  • Toll-Like Receptor 4
  • Toll-Like Receptors
  • Vaccines, DNA