Antisense suppression of collagen VI synthesis results in reduced expression of collagen I in normal human osteoblast-like cells

Biosci Biotechnol Biochem. 2002 Dec;66(12):2743-7. doi: 10.1271/bbb.66.2743.

Abstract

A transient increase in collagen VI expression precedes the accumulation of collagen I associated with interleukin-4 (IL-4)-induced mineralization in human osteoblast-like cells. Transfection with an antisense oligonucleotide specific for alpha1(VI) collagen mRNA was shown to attenuate mRNA levels of collagens VI and I. Incubating IL-4 treated cells with anti-collagen VI antiserum decreased expression of alpha1(I) mRNA. The results suggest that collagen VI may regulate collagen I expression in the early phase of IL-4-induced mineralization.

MeSH terms

  • Cells, Cultured
  • Collagen Type I / genetics*
  • Collagen Type VI / genetics*
  • Gene Silencing*
  • Humans
  • Interleukin-4 / pharmacology
  • Oligonucleotides, Antisense / metabolism*
  • Osteoblasts / metabolism*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism

Substances

  • Collagen Type I
  • Collagen Type VI
  • Oligonucleotides, Antisense
  • RNA, Messenger
  • Interleukin-4