Objective: To explore a simple and convenient immunoassay for early diagnosis of Taxoplasma infection.
Methods: Urine samples collected from three groups of mice infected with different doses of tachyzoites were detected for Taxoplasma circulating antigen (TCA) by dot-ELISA using HRP-SPA as a second antibody (SPA-ELISA).
Results: Taxoplasma circulating antigens were detected in all three groups of infected mice in contrast with the normal control group. Taxoplasma circulating antigen was detected on d6 and d3 after infection in mice of light- and moderate-infection groups, respectively.
Conclusion: SPA-ELISA is a simple and convenient method for early immunodiagnosis of recent Taxoplasma infection.