Analysis of reaction products of morphine and codeine with hydrogen peroxide by high-performance liquid chromatography/mass spectrometry

Anal Sci. 2003 Jan;19(1):163-5. doi: 10.2116/analsci.19.163.

Abstract

Changes in the chemical structures of morphine and codeine in the presence of hydrogen peroxide, a major component of hair dye and decolorant treatments, were examined with high-performance liquid chromatography/mass spectrometry (LC/MS). A mixture of morphine and hydrogen peroxide solution, after incubation at 39 degrees C for 24 h, produced two reaction products (hydroxymorphines). When codeine was used in place of morphine, one reaction product (hydroxycodeine) was produced, in which the benzene ring was hydroxylated.

MeSH terms

  • Chromatography, High Pressure Liquid / methods*
  • Codeine / chemistry*
  • Hydrogen Peroxide / chemistry*
  • Mass Spectrometry / methods*
  • Morphine / chemistry*

Substances

  • Morphine
  • Hydrogen Peroxide
  • Codeine