NF-kappaB inhibition impairs the radioresponse of hypoxic EMT-6 tumour cells through downregulation of inducible nitric oxide synthase

Br J Cancer. 2003 Jan 13;88(1):120-4. doi: 10.1038/sj.bjc.6600678.

Abstract

Hypoxic EMT-6 tumour cells displayed a high level of inducible nitric oxide synthase (iNOS) and an increased radiosensitivity after a 16 h exposure to lipopolysaccharide, a known activator of nuclear factor-kappaB (NF-kappaB). Both iNOS activation and radioresponse were impaired by the NF-kappaB inhibitors phenylarsine oxide and lactacystin. Contrasting to other studies, our data show that inhibition of NF-kappaB may impair the radioresponse of tumour cells through downregulation of iNOS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcysteine / analogs & derivatives*
  • Acetylcysteine / pharmacology*
  • Animals
  • Anti-Bacterial Agents / pharmacology
  • Arsenicals / pharmacology
  • Cell Hypoxia
  • Cysteine Endopeptidases / metabolism
  • Down-Regulation
  • Lactams
  • Mice
  • Multienzyme Complexes / metabolism
  • NF-kappa B / antagonists & inhibitors*
  • NF-kappa B / physiology
  • Nitric Oxide Synthase / metabolism*
  • Nitric Oxide Synthase Type II
  • Oxygen / metabolism
  • Proteasome Endopeptidase Complex
  • Radiation Tolerance / drug effects*
  • Radiation Tolerance / physiology
  • Tumor Cells, Cultured

Substances

  • Anti-Bacterial Agents
  • Arsenicals
  • Lactams
  • Multienzyme Complexes
  • NF-kappa B
  • oxophenylarsine
  • lactacystin
  • Nitric Oxide Synthase
  • Nitric Oxide Synthase Type II
  • Nos2 protein, mouse
  • Cysteine Endopeptidases
  • Proteasome Endopeptidase Complex
  • Oxygen
  • Acetylcysteine