Comparison of the TUNEL, lamin B and annexin V methods for the detection of apoptosis by flow cytometry

Acta Histochem. 2002;104(4):367-70. doi: 10.1078/0065-1281-00674.

Abstract

The present study was performed in order to compare the sensitivity of 3 apoptosis-detection methods using a model cell culture after induction of apoptosis. Evaluation was performed by means of flow cytometry and light microscopy. It appeared that both TUNEL and annexin V methods are sensitive and specific and produced similar data in all measurements. The immunocytochemical detection of lamin B was less reliable than the other methods.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Annexin A5* / analysis
  • Apoptosis / drug effects
  • Apoptosis / physiology*
  • DNA Fragmentation / drug effects
  • Dactinomycin / pharmacology
  • Enzyme Inhibitors / pharmacology
  • Etoposide / pharmacology
  • Flow Cytometry / methods*
  • Fluorescent Antibody Technique, Indirect
  • HL-60 Cells
  • Humans
  • In Situ Nick-End Labeling* / methods*
  • Kinetin
  • Lamin Type B* / analysis
  • Purines / pharmacology
  • Sensitivity and Specificity

Substances

  • Annexin A5
  • Enzyme Inhibitors
  • Lamin Type B
  • Purines
  • bohemine
  • Dactinomycin
  • olomoucine
  • Etoposide
  • Kinetin