Abstract
Using a different fragment DE6 obtained from DD-PCR as probe, a full-length cDNA has been cloned from human liver cDNA library and named as syap1 (Synapse-associated protein 1) because its deduced amino acid sequence is homologous to SAP47 of D. melanogaster. Northern blot analysis reveals that two transcripts of syap1 mRNA are expressed in cultured human liver L-02 cells. Moreover, the syap1 mRNA was also detected in most of adult human tissues by RT-PCR analysis. Down-regulated expression of syap1 mRNA was confirmed by semi-quantitative RT-PCR assay in 5 of 10 (50%) cases of human hepatocellular carcinoma (HCC).
Publication types
-
English Abstract
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adult
-
Amino Acid Sequence
-
Base Sequence
-
Carcinoma, Hepatocellular / genetics*
-
Carcinoma, Hepatocellular / metabolism
-
Carrier Proteins / biosynthesis
-
Carrier Proteins / genetics*
-
Cloning, Molecular
-
Gene Expression Regulation, Neoplastic
-
Humans
-
Liver Neoplasms / genetics*
-
Liver Neoplasms / metabolism
-
Molecular Sequence Data
-
Nerve Tissue Proteins / biosynthesis
-
Nerve Tissue Proteins / genetics*
-
RNA, Messenger / biosynthesis
-
RNA, Messenger / genetics
-
Reverse Transcriptase Polymerase Chain Reaction
-
Tumor Cells, Cultured
Substances
-
Carrier Proteins
-
Nerve Tissue Proteins
-
RNA, Messenger
-
Shank2 protein, rat