Abstract
Polymerase chain reaction was performed to screen Bacillus thuringiensis isolates for cryI, cryIII, and cryV genes with cryI-, cryIII-, and cryV-specific primers respectively. The results indicated that, in the 56 Bt isolates examined, 7 isolates contain cryI gene, 2 isolates contain cryIII gene, and 21 isolates contain both cryI and cryV genes. The cryV gene was located by Southern hybridization in the large plasmid of about 150 MD molecular mass.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacillus thuringiensis / chemistry
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Bacillus thuringiensis / genetics*
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Bacillus thuringiensis Toxins
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Bacterial Proteins / genetics*
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Bacterial Proteins / isolation & purification
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Bacterial Toxins / genetics*
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Bacterial Toxins / isolation & purification
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DNA Primers
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DNA, Bacterial / genetics
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Endotoxins / genetics*
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Endotoxins / isolation & purification
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Genes, Bacterial*
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Hemolysin Proteins
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Plasmids
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Polymerase Chain Reaction
Substances
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Bacillus thuringiensis Toxins
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Bacterial Proteins
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Bacterial Toxins
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DNA Primers
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DNA, Bacterial
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Endotoxins
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Hemolysin Proteins
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insecticidal crystal protein, Bacillus Thuringiensis