Isolation and structure of the cytotoxic cycloheptapeptide phakellistatin 13

J Nat Prod. 2003 Jan;66(1):146-8. doi: 10.1021/np020223y.

Abstract

A new cyclic heptapeptide phakellistatin 13 (1) had been isolated from the sponge Phakellia fusca Thiele, collected at Yongxing Island of China. Its structure was elucidated as cyclo-(Pro1-Trp-Leu-Thr-Pro2-Gly-Phe) on the basis of MS, UV, IR, and high-field NMR (600 MHz) analysis. The compound was significantly cytotoxic against the human hepatoma BEL-7404 cell line with an ED(50) < 10(-2) microg/mL.

MeSH terms

  • Animals
  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / isolation & purification*
  • Antineoplastic Agents / pharmacology
  • Carcinoma, Hepatocellular
  • China
  • Drug Screening Assays, Antitumor
  • Humans
  • Molecular Structure
  • Peptides, Cyclic / chemistry
  • Peptides, Cyclic / isolation & purification*
  • Peptides, Cyclic / pharmacology
  • Porifera / chemistry*
  • Tumor Cells, Cultured / drug effects

Substances

  • Antineoplastic Agents
  • Peptides, Cyclic
  • phakellistatin 13