In situ hybridization with Digoxingenin labeled probes were used to build the relationship between incised cutaneous wounding intervals and the expression of splicing fibronectin variants (total, E III A and E III B Variants) in rats. The results showed that all forms of FN variants increased notably with the wounding time going on. E III A and E III B Variants were expressed abundantly 30 and 60 minutes after injury, while few in normal skin.