Rapid detection of fungal filaments in corneal scrapings by microwave heating-assisted Grocott's methenamine silver staining

Indian J Ophthalmol. 2002 Dec;50(4):326-8.

Abstract

The Gomoris methanamine silver impregnation technique is a highly reliable and archiveable method of detecting fungal filaments, but the staining procedure is time consuming and laborious. A technique using microwave energy to reduce the duration of Gomori's silver staining is described.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cornea / microbiology*
  • Cornea / pathology
  • Eye Infections, Fungal / diagnosis
  • Eye Infections, Fungal / microbiology*
  • Fungi / isolation & purification
  • Heating / methods
  • Humans
  • Keratitis / diagnosis
  • Keratitis / microbiology*
  • Microwaves
  • Mycoses / diagnosis
  • Mycoses / microbiology*
  • Sensitivity and Specificity
  • Silver Staining / instrumentation
  • Silver Staining / methods*
  • Staining and Labeling / methods