Immunohistochemical investigations of neurofilament M' and alphabeta-crystallin in the magnocellular layers of the primate lateral geniculate nucleus

Brain Res Mol Brain Res. 2002 Dec 30;109(1-2):216-20. doi: 10.1016/s0169-328x(02)00564-8.

Abstract

The magnocellular and parvocellular pathways are two major processing streams in the primate visual system. Using high-density grid arrayed cDNA clones to hybridize to cDNA probes from cortical regions of each pathway, a list of candidate differentially expressed genes was produced [Mol. Brain Res. 82 (2000) 11-24]. Magnocellular pathway candidates include neurofilament M' and alphabeta-crystallin. Using antibodies generated against these proteins, immunohistochemical analysis revealed preferential staining of the magnocellular layers in the primate lateral geniculate nucleus, providing verification of two candidate magnocellular-enriched genes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Geniculate Bodies / metabolism*
  • Immunohistochemistry
  • Macaca fascicularis
  • Macaca mulatta
  • Neurofilament Proteins / metabolism*
  • Neurons / cytology
  • Neurons / metabolism*
  • Visual Pathways / metabolism
  • alpha-Crystallin B Chain / metabolism*

Substances

  • Neurofilament Proteins
  • alpha-Crystallin B Chain
  • neurofilament protein M