Objective: To establish an Ehrlich reaction-colorimetry method for determining furostanol saponins in Bulbus Allii Macroste.
Method: Based on the coloration reaction between furostanol saponins and p-dimethylaminobenzaldehyde, the UV detection wavelength used was 515 nm, and the optimum conditions were selected on homogeneous design principle.
Result: The standard curve of macrostemonoside G was linear in the concentration range of 0.1975-0.9880 mg.ml-1. The average recovery was 97.7% (RSD = 3.65%).
Conclusion: The method is rapid, accurate and easy to operate.