Effect of a combination therapy between IL-12 and soluble IL-4 receptor (sIL-4R) on Candida albicans and herpes simplex virus type I infections in thermally injured mice

Can J Microbiol. 2002 Oct;48(10):886-94. doi: 10.1139/w02-085.

Abstract

The effectiveness of a combination using IL-12 and soluble IL-4 receptor (sIL-4R) to treat severe infections of herpes simplex virus type 1 (HSV-1) and Candida albicans in thermally injured mice was investigated. Although sIL-4R decreased burn-associated type 2 T-cell responses, the effect of sIL-4R was minimal on the morbidity and mortality of thermally injured mice exposed to 250 times LD50 of HSV-1 or 10 times LD50 of C. albicans. Compared with 100% mortality in control mice, mortality for HSV-1 and C. albicans was 40 and 20%, respectively, in thermally injured mice that received IL-12 and sIL-4R in combination. After stimulation with anti-CD3 monoclonal antibody, splenic T cells from thermally injured mice exposed to large amounts of HSV-1 or C. albicans did not produce gamma interferon (IFN-gamma) into their culture fluids. However, IFN-gamma was produced by splenic T cells from thermally injured and infected mice treated with IL-12 and sIL-4R in combination. These results suggest that therapeutic treatment with a combination of IL-12 and sIL-4R may be effective by inducing type 1 T-cell responses in thermally injured mice exposed to large amounts of HSV-1 or C. albicans.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Burns / complications*
  • Burns / immunology
  • Burns / microbiology
  • Candida albicans / drug effects
  • Candida albicans / immunology
  • Candidiasis / drug therapy*
  • Drug Therapy, Combination
  • Herpes Simplex / drug therapy*
  • Herpes Simplex / immunology
  • Herpesvirus 1, Human / drug effects
  • Interleukin-12 / therapeutic use*
  • Interleukin-4 / blood
  • Macrophages / microbiology
  • Mice
  • Mice, Inbred BALB C
  • Receptors, Interleukin-4 / therapeutic use*
  • Spleen
  • T-Lymphocytes / immunology
  • Treatment Outcome

Substances

  • Receptors, Interleukin-4
  • Interleukin-12
  • Interleukin-4