Development of supercritical fluid extraction with a solid-phase trapping for fast estimation of toxic load of polychlorinated dibenzo-p-dioxins-dibenzofurans in sawmill soil

J Chromatogr A. 2002 Oct 25;975(1):189-98. doi: 10.1016/s0021-9673(02)01325-0.

Abstract

A method consisting of automated supercritical fluid extraction (SFE) with simultaneous cleanup by a solid-phase trap was developed for fast analysis of polychlorinated dibenzo-p-dioxins (PCDDs) and dibenzofurans (PCDFs) in soil. SFE was optimised to replace conventional liquid-based methods in routine analyses of PCDD/PCDFs in sawmill soil contaminated by a chlorophenol formulation. PCDD/PCDFs were quantitatively extracted in 60 min using CO2 at 400 atm and 100 degrees C without a modifier. A trap containing a small amount of activated carbon mixed with Celite efficiently collected PCDD/PCDFs after SFE. After SFE co-extracted impurities were eluted out from the trap with 4 ml of hexane and PCDD/PCDFs were eluted with 10 ml of toluene. The concentrations and TCDD-equivalent of PCDD/PCDFs corresponded to the results of traditional solvent extraction method (Soxhlet) in six sawmill soils tested. The performance of the trap was maintained over a long period of time (nearly 100 extractions).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzofurans / analysis*
  • Dibenzofurans, Polychlorinated
  • Polychlorinated Dibenzodioxins / analogs & derivatives*
  • Polychlorinated Dibenzodioxins / analysis*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Soil Pollutants / analysis*

Substances

  • Benzofurans
  • Dibenzofurans, Polychlorinated
  • Polychlorinated Dibenzodioxins
  • Soil Pollutants