Abstract
In this study we have established conditions for p65 gene expression analysis by reverse transcriptase polymerase chain reaction (RT-PCR). On the basis of this technique we analyzed p65 gene expression in various types of leukemia: acute myeloblastic leukemia (AML) (n=26); acute lymphoblastic leukemia (ALL) (n=26) and chronic lymphocytic leukemia (CLL) (n=40). The highest frequency of p65 gene expression was found in the patients with CLL (66%). No relationship between the expression of p65 gene and clinical stage of leukemia was observed. The lower percentage of positivity (presence of gene transcript) was seen in patients with ALL (42%) and AML (46%).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Adult
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Aged
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Aged, 80 and over
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Biomarkers, Tumor*
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Bone Marrow Cells / metabolism
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Carrier Proteins / biosynthesis*
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Carrier Proteins / genetics
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DNA, Complementary / metabolism
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Female
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Humans
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Intracellular Signaling Peptides and Proteins
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Leukemia, Lymphocytic, Chronic, B-Cell / blood*
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Male
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Middle Aged
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Neoplasm Proteins / biosynthesis*
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Neoplasm Proteins / genetics
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Precursor Cell Lymphoblastic Leukemia-Lymphoma / blood*
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Reverse Transcriptase Polymerase Chain Reaction
Substances
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Biomarkers, Tumor
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Carrier Proteins
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DNA, Complementary
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HSAJ2425 protein, human
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Intracellular Signaling Peptides and Proteins
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Neoplasm Proteins