Bollworm responses to release of genetically modified Helicoverpa armigera nucleopolyhedroviruses in cotton

J Invertebr Pathol. 2002 Oct;81(2):63-9. doi: 10.1016/s0022-2011(02)00144-1.

Abstract

Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (HaSNPV) has been developed as a commercial biopesticide to control the cotton bollworm, H. armigera, in China. The major limitation to a broader application of this virus has been the relative long time to incapacitate the target insect. Two HaSNPV recombinants with improved insecticidal properties were released in bollworm-infested cotton. One recombinant (HaCXW1) lacked the ecdysteroid UDP-glucosyltransferase (egt) gene and in another recombinant (HaCXW2), an insect-selective scorpion toxin (AaIT) gene replaced the egt gene. In a cotton field situation H. armigera larvae treated with either HaCXW1 or HaCXW2 were killed faster than larvae in HaSNPV-wt treated plots. Second instar H. armigera larvae, which were collected from HaCXW1 and HaCXW2 treated plots and further reared on artificial diet, showed reduced ST(50) values of 15.3 and 26.3%, respectively, as compared to larvae collected from HaSNPV-wt treated plots. The reduction in consumed leaf area of field collected larvae infected with HaCXW1 and HaCXW2 was approximated 50 and 63%, respectively, as compared to HaSNPV-wt infected larvae at 108 h after treatment. These results suggest that in a cotton field situation the recombinants will be more effective control agents of the cotton bollworm than wild-type HaSNPV.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA, Recombinant / genetics*
  • Genetic Engineering*
  • Gossypium / virology
  • Larva / virology
  • Moths / virology*
  • Nucleopolyhedroviruses / genetics*
  • Pest Control, Biological / methods*
  • Polymerase Chain Reaction
  • Scorpion Venoms / genetics
  • Time Factors

Substances

  • DNA, Recombinant
  • Scorpion Venoms
  • AaIT neurotoxin, Androctonus australis