Microbial transformation of rubijervine

Chem Pharm Bull (Tokyo). 2002 Nov;50(11):1427-9. doi: 10.1248/cpb.50.1427.

Abstract

Preparative-scale fermentation of rubijervine (1), the known 22,26-epiminocholestane Veratrum alkaloid, with Cunninghamella echinulata ATCC 9244 has resulted in the isolation of the new metabolites 7alpha-hydroxyrubijervine (2) and solanid-5-ene-3beta,12alpha-diol-1-one (3). Structure elucidation of these metabolites was based primarily on 1D- and 2D-NMR analyses. The microbe C. echinulata ATCC 9244 was able to metabolize rings A and B of rubijervine but failed to metabolize rings C, D or its N-containing side chain, a finding which is analogous to the results of previous fermentation studies of steroidal alkaloids.

MeSH terms

  • Antihypertensive Agents / chemistry
  • Antihypertensive Agents / pharmacokinetics
  • Biotransformation / physiology
  • Cunninghamella / metabolism*
  • Fermentation
  • Veratrum Alkaloids / chemistry*
  • Veratrum Alkaloids / pharmacokinetics*

Substances

  • Antihypertensive Agents
  • Veratrum Alkaloids