Mutation analysis of CBP and PCAF reveals rare inactivating mutations in cancer cell lines but not in primary tumours

Br J Cancer. 2002 Nov 4;87(10):1162-5. doi: 10.1038/sj.bjc.6600554.

Abstract

In this study we screened the histone acetyltransferases CBP and PCAF for mutations in human epithelial cancer cell lines and primary tumours. We identified two CBP truncations (both in cell lines), seven PCAF missense variants and four CBP intronic microdeletions. These data suggest that neither gene is commonly inactivated in human epithelial cancers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetyltransferases / genetics*
  • Blotting, Western
  • CREB-Binding Protein
  • Cell Cycle Proteins / genetics
  • Exons
  • Histone Acetyltransferases
  • Humans
  • Introns
  • Mutation*
  • Neoplasms, Glandular and Epithelial / genetics*
  • Nuclear Proteins / genetics*
  • Saccharomyces cerevisiae Proteins / genetics*
  • Trans-Activators / genetics*
  • Transcription Factors
  • Tumor Cells, Cultured
  • p300-CBP Transcription Factors

Substances

  • Cell Cycle Proteins
  • Nuclear Proteins
  • Saccharomyces cerevisiae Proteins
  • Trans-Activators
  • Transcription Factors
  • Acetyltransferases
  • CREB-Binding Protein
  • CREBBP protein, human
  • Histone Acetyltransferases
  • p300-CBP Transcription Factors
  • p300-CBP-associated factor