Isolation and characterisation of nuclear mutants with enhanced mitochondrial mutability in the fission yeast Schizosaccharomyces pombe

Microbiol Res. 2002;157(3):197-200. doi: 10.1078/0944-5013-00151.

Abstract

In this paper we report the isolation and preliminary characterisation of nuclear mutants with increased mitochondrial mutability in fission yeast. Screening of about 2000 clones after nitrosoguanidine mutagenesis led to the isolation of ten mutator mutants. For one of them (mut-1) we show that the mutation is chromosomally encoded. The activity of the mutator is restricted to the mitochondrial genome, since it increases the mutation rate to mitochondrially encoded drug resistance considerably, whereas the mutability of nuclear genes is not altered.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Nucleus / genetics*
  • Cytochrome b Group / metabolism
  • DNA, Mitochondrial / genetics*
  • Diuron / metabolism
  • Diuron / pharmacology
  • Drug Resistance, Fungal
  • Mutation
  • Schizosaccharomyces / drug effects
  • Schizosaccharomyces / genetics*
  • Schizosaccharomyces / isolation & purification

Substances

  • Cytochrome b Group
  • DNA, Mitochondrial
  • Diuron