Abstract
Aim:
To study the protective effects of Ginkgo biloba extract (GbE) against rat aortic endothelial cells (RAEC) damage induced by lysophosphatidylcholine (LPC).
Methods:
Cell injury were determined by MTT assay and LDH release. Vascular endothelial growth factor (VEGF) protein production from RAEC was determined by enzyme-linked immunosorbent assay (ELISA). VEGF mRNA expression was examined by in situ hybridization and dot blot.
Results:
GbE 0.01-1 microg/L prevented LPC-induced injury in cultured RAEC in a concentration-dependent manner. Cultured RAEC could express VEGF protein and VEGF mRNA was induced by LPC 5 mg/L. GbE could inhibit the expression of VEGF protein and VEGF mRNA in co-cultured RAEC with LPC.
Conclusion:
LPC could induce a strong expression of VEGF in RAEC. GbE could protect RAEC against the LPC-induced damage and downregulate VEGF protein and VEGF mRNA expression in cultured RAEC.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Aorta / cytology
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Aorta / metabolism
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Cells, Cultured
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Drugs, Chinese Herbal / pharmacology*
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Endothelial Growth Factors / genetics
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Endothelial Growth Factors / metabolism*
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Endothelium, Vascular / drug effects*
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Endothelium, Vascular / metabolism
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Endothelium, Vascular / pathology
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Enzyme-Linked Immunosorbent Assay
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Ginkgo biloba / chemistry*
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Intercellular Signaling Peptides and Proteins / genetics
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Intercellular Signaling Peptides and Proteins / metabolism*
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Lymphokines / genetics
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Lymphokines / metabolism*
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Lysophosphatidylcholines
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Male
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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Rats
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Rats, Wistar
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Vascular Endothelial Growth Factor A
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Vascular Endothelial Growth Factors
Substances
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Drugs, Chinese Herbal
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Endothelial Growth Factors
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Intercellular Signaling Peptides and Proteins
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Lymphokines
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Lysophosphatidylcholines
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RNA, Messenger
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Vascular Endothelial Growth Factor A
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Vascular Endothelial Growth Factors