Disruption of protein-protein interactions: design of a synthetic receptor that blocks the binding of cytochrome c to cytochrome c peroxidase

Chem Commun (Camb). 2001 Sep 7:(17):1580-1. doi: 10.1039/b104142h.

Abstract

Synthetic receptor 1 has been found via fluorescence titration to compete effectively with cytochrome c peroxidase for binding cytochrome c (Cc), forming 1:1 Cc:1 complex with a binding constant of (3 +/- 1) x 10(8) M-1, and to disrupt Cc: Apaf-1 complex, a key adduct in apoptosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / drug effects
  • Apoptotic Protease-Activating Factor 1
  • Binding, Competitive / drug effects
  • Carrier Proteins / chemical synthesis*
  • Carrier Proteins / metabolism
  • Carrier Proteins / pharmacology*
  • Cytochrome c Group / antagonists & inhibitors*
  • Cytochrome c Group / metabolism
  • Cytochrome-c Peroxidase / antagonists & inhibitors*
  • Cytochrome-c Peroxidase / metabolism
  • Protein Binding / drug effects
  • Protein Conformation
  • Proteins / antagonists & inhibitors
  • Proteins / metabolism
  • Spectrometry, Fluorescence
  • Thermodynamics

Substances

  • Apoptotic Protease-Activating Factor 1
  • Carrier Proteins
  • Cytochrome c Group
  • Proteins
  • Cytochrome-c Peroxidase