Survey of human papillomavirus infection in cervical intraepithelial neoplasia in Thai women

J Med Assoc Thai. 2002 Jun:85 Suppl 1:S360-5.

Abstract

HPV infection is known to be associated with cervical cancer development. Precancerous lesions named cervical intraepithelial neoplasia (CIN) are divided into 3 grades, i.e., CIN-1, CIN-2, and CIN-3. Here, HPV infection determined by PCR and dot hybridization was observed in these 3 different grades of formalin-fixed paraffin embedded tissues. The HPV infection was demonstrated in 33.3 per cent of CIN-1, 36.8 per cent of CIN-2 and 75 per cent of CIN-3. Using type specific probes for HPV-6, 11, 16, 18 and 33, HPV-16 was the most prevalent type (44.44%) followed by HPV-18 (16.05%) in CIN-3. Only one HPV-18 was identified in CIN-1 while CIN-2 contained one HPV-6 and one HPV-18. Mixed infection was found in CIN-3 (12.35%). All of them had HPV-16. The cervicitis cases with normal histopathology were included as control. Only 2.7 per cent of HPV infection was shown. The relative risk of HPV infection was high in CIN-3 (OR = 107.25, 95% CI = 50.29-228.73). Our data confirm the association between high-risk HPV types and development of CIN.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Biopsy, Needle
  • Case-Control Studies
  • Chi-Square Distribution
  • Confidence Intervals
  • Data Collection
  • Female
  • Humans
  • Immunohistochemistry
  • Middle Aged
  • Odds Ratio
  • Papillomaviridae / isolation & purification*
  • Papillomavirus Infections / epidemiology
  • Papillomavirus Infections / pathology*
  • Polymerase Chain Reaction
  • Precancerous Conditions / pathology
  • Reference Values
  • Sensitivity and Specificity
  • Thailand / epidemiology
  • Tumor Virus Infections / epidemiology
  • Tumor Virus Infections / pathology*
  • Uterine Cervical Dysplasia / epidemiology
  • Uterine Cervical Dysplasia / microbiology
  • Uterine Cervical Dysplasia / pathology*
  • Uterine Cervical Neoplasms / epidemiology
  • Uterine Cervical Neoplasms / microbiology
  • Uterine Cervical Neoplasms / pathology*