Molecular cloning and expression analysis of MPP alpha-2, a novel mouse transcript detected in a differential screen of pituitary libraries

Biochim Biophys Acta. 2002 Aug 19;1577(1):109-12. doi: 10.1016/s0167-4781(02)00293-2.

Abstract

We identified a novel isoform transcript, MPP alpha-2, of the mouse Mg(2+)-dependent protein phosphatase (MPP) alpha gene. The amino acid sequence encoded by MPP alpha-2 differs from the previously known MPP alpha-1 sequence only at the carboxyl terminal region. Northern and in situ hybridization analysis revealed differential expression patterns of these two transcripts in the embryo and in the adult organism, suggesting an elaborate regulation of the MPP alpha gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Gene Expression Profiling
  • Gene Library
  • Mice
  • Molecular Sequence Data
  • Phosphoprotein Phosphatases / biosynthesis*
  • Phosphoprotein Phosphatases / genetics*
  • Pituitary Gland / embryology
  • Pituitary Gland / enzymology*
  • Protein Phosphatase 2C
  • RNA, Messenger / analysis
  • Sequence Homology, Amino Acid
  • Tissue Distribution
  • Transcription, Genetic

Substances

  • RNA, Messenger
  • Phosphoprotein Phosphatases
  • Protein Phosphatase 2C

Associated data

  • GENBANK/AF369981