Objective: To determine the role of GATA-3 (a transcript factor that has influences on Th2 cell differentiation) in the development of anterior chamber associated immune deviation (ACAID).
Method: Immunohistochemistry and Western blot were used to determine the localization and relative protein levels of GATA-3 respectively at different time points after anterior chamber injection of 50 microgram interphotoreceptor retinoid binding protein (IRBP).
Result: GATA-3 was detected weakly in normal spleen and increased significantly at 5, 7, 14 and 21 days after anterior chamber inoculation. It was detected mainly in CD(4)(+) T cells in spleen.
Conclusion: The expression of GATA-3 increases earlier than development of ACAID. It suggests that GATA-3 play an important role in Th2 commitment during ACAID development.