A modified molecular beacon combining the properties of TaqMan probe

Chem Commun (Camb). 2002 Apr 21:(8):854-5. doi: 10.1039/b200592a.

Abstract

A modified molecular beacon that possesses a stem-hairpin structure as seen in conventional molecular beacons and can be cleaved during PCR in designed, and it can specifically recognize the presence of the target and was obviously more sensitive than conventional molecular beacons.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / chemistry
  • DNA / metabolism*
  • DNA Probes / chemistry
  • Fluorescent Dyes / chemistry
  • Hepatitis B virus / genetics*
  • Hepatitis B virus / metabolism
  • Humans
  • Oligonucleotides / chemistry
  • Oligonucleotides / metabolism*
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity

Substances

  • DNA Probes
  • Fluorescent Dyes
  • Oligonucleotides
  • DNA