An NF-IL6 3'UTR RNA-specific Binding Protein in E. coli Purification and Partial Protein Sequencing

Guo-Liang Qing; Hu-Dan Liu; Ding-Gan Liu

An NF-IL6 3'UTR RNA-specific Binding Protein in E. coli Purification and Partial Protein Sequencing

Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2000;32(1):49-54.

Authors

Guo-Liang Qing¹, Hu-Dan Liu, Ding-Gan Liu

Affiliation

¹ Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. liudiga@sunm.shcnc.ac.cn

PMID: 12110913

Abstract

protein was found in E.coli which can specifically bind to NF-IL6 mRNA 3'UTR. After a series of purification steps, the RNA-specific binding protein was directly sequenced on the Porton LF3200 Protein Sequencer. A sequence of 10 amino acids of the purified NF-IL6 3'UTR binding protein was obtained, namely, Ala-Thr-Arg-Ile-Glu-Phe-His-Gly-Cyss(?)-Gly. A BLAST search with the 10 amino acids against NCBI database failed to identify any protein with identical sequence. The significance of identifying an eukaryotic mRNA binding protein in E.coli is discussed.