The effects of ethanol on glucose 6-phosphate dehydrogenase enzyme activity from human erythrocytes in vitro and rat erythrocytes in vivo

Alcohol Alcohol. 2002 Jul-Aug;37(4):327-9. doi: 10.1093/alcalc/37.4.327.

Abstract

Aims: The effects of ethanol on erythrocyte glucose 6-phosphate dehydrogenase (G6PD) activity were investigated under in vitro and in vivo conditions.

Methods: For in vitro studies, glucose 6-phosphate dehydrogenase was purified from human erythrocyte and rats were used for in vivo studies. Enzyme activity was determined spectrophotometrically by the Beutler method.

Results: The in vitro study showed that the I(50) value was 17 mM for ethanol. In the case of the in vivo study, a 2 ml/kg dose of ethanol significantly inhibited the G6PD activity. The inhibition rate after ethanol administration was 59%, 40% and 6% at 1, 3 and 6 h after, respectively.

Conclusions: The results of this study suggest that ethanol has a significant inhibitory effect on the G6PD activity both in vivo and in vitro.

MeSH terms

  • Animals
  • Enzyme Activation / drug effects
  • Erythrocytes / drug effects*
  • Erythrocytes / enzymology
  • Ethanol / pharmacology*
  • Glucosephosphate Dehydrogenase / metabolism*
  • Humans
  • In Vitro Techniques
  • Male
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Ethanol
  • Glucosephosphate Dehydrogenase