Increased interleukin 10, tumor necrosis factor alpha, and interleukin 6 levels in blister fluid of toxic epidermal necrolysis

J Am Acad Dermatol. 2002 Jul;47(1):58-62. doi: 10.1067/mjd.2002.120473.

Abstract

Background: Toxic epidermal necrolysis is a severe, usually drug-induced disease that shares clinical, histologic, and immunologic similarities with the severe forms of cutaneous acute graft-versus-host disease.

Objective: Our purpose was to further characterize common immune-inflammatory pathways in these skin disorders by measurement of different cytokines.

Methods: Evaluation of serum levels of interleukin 10 (IL-10), tumor necrosis factor alpha, IL-6, and soluble IL-6 receptor in the early phase of both diseases and in blister fluid of toxic epidermal necrolysis.

Results: Serum levels of IL-10 and IL-6 were significantly higher in patients with toxic epidermal necrolysis (P =.0001) and acute graft-versus-host disease (P =.001) compared with those of blood donors. We found an increase in IL-6 levels in blister fluid and significantly higher levels of IL-10 (P =.018) and tumor necrosis factor alpha (P =.028) in blister fluid compared with serum in patients with toxic epidermal necrolysis.

Conclusion: A similar serum cytokine profile of toxic epidermal necrolysis and acute graft-versus-host disease further emphasizes common immunologic mechanisms. The presence of inflammatory cytokines, IL-6 and tumor necrosis factor alpha, in the blister fluid of patients with toxic epidermal necrolysis is associated with significantly higher levels of IL-10, which through its down-regulatory role, may be involved in limitation of the disease extension.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers / analysis
  • Blister / metabolism
  • Female
  • Graft vs Host Disease / physiopathology*
  • Humans
  • Inflammation Mediators / analysis*
  • Interleukin-10 / analysis*
  • Interleukin-10 / metabolism
  • Male
  • Middle Aged
  • Probability
  • Prospective Studies
  • Sampling Studies
  • Sensitivity and Specificity
  • Statistics, Nonparametric
  • Stevens-Johnson Syndrome / physiopathology*
  • Tumor Necrosis Factor-alpha / analysis*
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Biomarkers
  • Inflammation Mediators
  • Tumor Necrosis Factor-alpha
  • Interleukin-10