Purification and initial crystallization studies of a DnaB intein from Synechocystis sp. PCC 6803

Xuehui Chen; Ming Qun Xu; Yi Ding; Sébastien Ferrandon; Zihe Rao

doi:10.1107/s0907444902006583

Purification and initial crystallization studies of a DnaB intein from Synechocystis sp. PCC 6803

Acta Crystallogr D Biol Crystallogr. 2002 Jul;58(Pt 7):1201-3. doi: 10.1107/s0907444902006583. Epub 2002 Jun 20.

Authors

Xuehui Chen¹, Ming Qun Xu, Yi Ding, Sébastien Ferrandon, Zihe Rao

Affiliation

¹ Laboratory of Structural Biology, Department of Biological Science and Technology and MOE Laboratory of Protein Science, Tsinghua University, Beijing 100084, People's Republic of China.

PMID: 12077443
DOI: 10.1107/s0907444902006583

Abstract

A 154-residue mini-intein from the dnaB gene of Synechocystis sp. PCC 6803 (Ssp DnaB intein) has been purified and crystallized using PEG 4000 as a precipitant. The crystal belongs to space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 58.2, c = 70.3 A. It has one molecule per asymmetric unit and diffracts to beyond 2.0 A under cryoconditions (100 K) using a rotating copper anode X-ray generator.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins*
Copper
Crystallography, X-Ray / methods*
Cyanobacteria / chemistry*
DNA Helicases / chemistry*
DNA Helicases / isolation & purification
DnaB Helicases
Electrodes
Electrophoresis, Polyacrylamide Gel
Plasmids / metabolism
Spectrophotometry, Atomic
Temperature

Substances

Bacterial Proteins
Copper
DNA Helicases
DnaB Helicases