Abstract
In this report, we describe the cloning, sequencing, and expression of the bovine orthologue of CD26 (BoCD26). Several monoclonal antibodies specific for a molecule, activation molecule 3 (ACT3), aberrantly expressed on superantigen-stimulated bovine CD8(+) lymphocytes, reacted with recombinant BoCD26 expressed in COS-7 and CHO cells. We also showed that human CD8(+) T cells stimulated by a superantigen expressed CD26 at high levels. These results demonstrate that ACT3 is identical to BoCD26 and suggest that CD26 upregulation on CD8(+) T cells is a general phenomenon of superantigens and not limited to their effects on bovine cells.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Antigens, Bacterial / immunology
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Base Sequence
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CD8-Positive T-Lymphocytes / immunology
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CHO Cells
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COS Cells
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Cattle
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Cells, Cultured
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Cloning, Molecular
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Cricetinae
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Dipeptidyl Peptidase 4 / biosynthesis*
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Dipeptidyl Peptidase 4 / genetics*
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Enterotoxins / immunology*
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Humans
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Molecular Sequence Data
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Sequence Alignment
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Staphylococcus aureus / immunology
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Superantigens / immunology*
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Up-Regulation
Substances
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Antigens, Bacterial
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Enterotoxins
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Superantigens
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enterotoxin C, staphylococcal
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Dipeptidyl Peptidase 4