Detection of CMV in plasma and aqueous humor specimens from AIDS patients with CMV retinitis using the amplicor CMV test

Scand J Infect Dis. 2002;34(5):351-4. doi: 10.1080/00365540110077489.

Abstract

A DNA hybridization test (the Amplicor CMV test) for CMV DNA was used to detect CMV DNA in paired plasma and aqueous humor specimens from 37 AIDS patients with ophthalmoscopically diagnosed CMV retinitis. CMV was detected in 25/37 (67.6%) plasma specimens and in 32/37 (86.5%) aqueous humor specimens. Thus, the Amplicor CMV test using aqueous humor specimens seems to be a useful diagnostic test for CMV retinitis and one that is more sensitive than determination of CMV DNA in plasma specimens.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • AIDS-Related Opportunistic Infections / diagnosis
  • AIDS-Related Opportunistic Infections / virology*
  • Aqueous Humor / virology*
  • Cytomegalovirus / genetics
  • Cytomegalovirus / isolation & purification*
  • Cytomegalovirus Retinitis / diagnosis
  • Cytomegalovirus Retinitis / virology*
  • DNA, Viral / analysis
  • DNA, Viral / blood*
  • Humans
  • Nucleic Acid Hybridization / methods
  • Reagent Kits, Diagnostic
  • Sensitivity and Specificity

Substances

  • DNA, Viral
  • Reagent Kits, Diagnostic