The photosynthetic reaction center (RC) composite film was fabricated by self-assembled monolayers (SAMs) on the Au electrode with two different bifunctional reagents, 4-aminothiophenol (ATP) and 2-mercaptoethylamine (MEA), respectively. The square wave voltametry (SWV), bulk electrolysis and photocurrent test were employed for characterizing the composite film. The dramatic different electrochemical characteristics were observed for the two types of films, which strongly suggested an orientational difference for RC arising from the structural difference between the two bifunctional reagents. For RC-MEA film, three redox peaks which implying electron transfer (ET) between the primary donor (P) and the bacteriopheophytin (Bphe) were observed. While for RC-ATP film, two redox peaks implying ET between the nonheme iron and the primary quinone (Q(A)) were observed. The ET behavior driven by electric field also supported the result that the RC could be linked to the electrode at different sites. The site-specific immobilization approach reported here supplies a method to differentiate the protein orientation.