Direct detection of Helicobacter pylori mutations associated with macrolide resistance in gastric biopsy material taken from human immunodeficiency virus-infected subjects

J Clin Microbiol. 2002 Jun;40(6):2234-7. doi: 10.1128/JCM.40.6.2234-2237.2002.

Abstract

One hundred forty gastric biopsies were tested by microbiological methods and by amplifying a sequence of 23S rRNA and identifying mutations associated to clarithromycin resistance. Seventy-six specimens were positive for Helicobacter pylori. Mutational analysis revealed alterations in 18 (39.1%) of 46 and 2 (8.7%) of 23 samples from human immunodeficiency virus-seropositive and -seronegative persons, respectively. The results of the mutational analysis fully correlated with those of the susceptibility tests.

Publication types

  • Evaluation Study

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Biopsy
  • Clarithromycin / pharmacology*
  • DNA, Ribosomal / analysis
  • Drug Resistance, Bacterial / genetics*
  • Electrophoresis, Polyacrylamide Gel / methods
  • Female
  • HIV Infections / complications*
  • HIV Infections / microbiology
  • Helicobacter Infections / microbiology
  • Helicobacter pylori / drug effects*
  • Helicobacter pylori / genetics
  • Helicobacter pylori / isolation & purification
  • Humans
  • Male
  • Microbial Sensitivity Tests
  • Mutation*
  • RNA, Ribosomal, 23S / genetics
  • Stomach

Substances

  • Anti-Bacterial Agents
  • DNA, Ribosomal
  • RNA, Ribosomal, 23S
  • Clarithromycin