In situ detection of cellular and abnormal isoforms of prion protein in brains of cattle with bovine spongiform encephalopathy and sheep with scrapie by use of a histoblot technique

J Vet Diagn Invest. 2002 May;14(3):255-7. doi: 10.1177/104063870201400313.

Abstract

To detect prion protein, brains from 5 cattle naturally affected with bovine spongiform encephalopathy (BSE) and 3 sheep naturally affected with scrapie were examined and compared with brains of normal cattle and sheep using a histoblot technique. The technique enabled the in situ distinctive detection of the cellular (PrP(C)) and abnormal (PrP(Sc)) isoforms of the prion protein. In BSE- or scrapie-affected brains, the Prp(C) signal decreased, especially in those areas where the PrP(Sc) signal was detected.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / pathology
  • Cattle
  • Encephalopathy, Bovine Spongiform / diagnosis*
  • Encephalopathy, Bovine Spongiform / pathology
  • Immunohistochemistry / methods*
  • Prions / analysis*
  • Protein Isoforms / analysis
  • Scrapie / diagnosis*
  • Scrapie / pathology
  • Sensitivity and Specificity
  • Sheep

Substances

  • Prions
  • Protein Isoforms