Factors affecting the adsorption of buchnericin LB, a bacteriocin produced by Lactobacillus [correction of Lactocobacillus] buchneri

Microbiol Res. 2002;157(2):103-7. doi: 10.1078/0944-5013-00134.

Abstract

Buchnericin-LB adsorbs to gram-positive but not to gram-negative bacteria. The tested gram-positive bacteria were species of Lactobacillus, Pediococcus, Leuconostoc, Enterococcus, Lactococcus, Listeria, Bacillus, Staphylococcus; gram-negative bacteria belonged to the genera Salmonella, Escherichia, Yersinia and Pseudomonas. Buchnericin-LB adsorption depended on pH but not on time and temperature. Also some anions of salts and lipoteichoic acid reduced or inhibited its adsorption. Treatment of cells and cell walls of sensitive bacteria with detergents, organic solvents or enzymes did not affect subsequent binding of buchnericin-LB. Treatment with buchnericin-LB caused sensitive cells to lose high amounts of intracellular K+ ions and UV-absorbing materials and became more permeable to o-nitrophenol-beta-D-galactopyranoside. Buchnericin-LB (640-2560 AU/ml) decreased the colony forming units (99%) and absorbance values of Listeria monocytogenes and Bacillus cereus. These results indicate that the mode of action of buchnericin-LB is bactericidal and its lethal effect is very rapid.

MeSH terms

  • Adsorption / drug effects
  • Bacillus cereus / drug effects
  • Bacillus cereus / growth & development
  • Bacteriocins / metabolism
  • Bacteriocins / pharmacokinetics*
  • Cell Membrane Permeability / drug effects
  • Cell Wall / drug effects
  • Cell Wall / metabolism
  • Colony Count, Microbial / methods
  • Gram-Negative Bacteria / drug effects
  • Gram-Negative Bacteria / growth & development
  • Gram-Positive Bacteria / drug effects
  • Gram-Positive Bacteria / growth & development
  • Lactobacillus / growth & development
  • Lactobacillus / metabolism*
  • Lactobacillus / radiation effects
  • Lipopolysaccharides / pharmacology
  • Listeria monocytogenes / drug effects
  • Listeria monocytogenes / growth & development
  • Nitrophenylgalactosides / pharmacology
  • Potassium / metabolism
  • Teichoic Acids / pharmacology
  • Ultraviolet Rays

Substances

  • Bacteriocins
  • Lipopolysaccharides
  • Teichoic Acids
  • buchnericin LB
  • 2-nitrophenylgalactoside
  • Nitrophenylgalactosides
  • lipoteichoic acid
  • Potassium