[Molecular and genetic polymorphism of cultivated soybean detected by AP-PCR, SSRP and SSR]

Tsitol Genet. 2001 Sep-Oct;35(5):3-9.
[Article in Russian]

Abstract

Molecular-genetic polymorphism of cultivated soybean from different geographic regions was analysed using three PCR methods (AP-PCR, SSRP and ISSR). The dendrogram of phenogenetic relationship was constructed on the base of obtained data. The allocation of varieties are in a good agreement with pedigree information. Main parameters such as polymorphism level, expected heterozygosity, and marker index were calculated. It was shown that ISSR is the method of choice for the differentiation of soybean varieties.

Publication types

  • English Abstract

MeSH terms

  • DNA, Plant / analysis
  • Glycine max / genetics*
  • Microsatellite Repeats
  • Phylogeny
  • Polymerase Chain Reaction / methods
  • Polymorphism, Genetic*
  • Repetitive Sequences, Nucleic Acid
  • Species Specificity
  • Ukraine

Substances

  • DNA, Plant