Thirty-eight patients with isthmic(31 cases), degenerative(6 cases), and post-traumatic(1 case) spondylolisthesis were treated with RF-II pedicle screw system. Eighteen patients were classified as Degree I lesion, 14 as Degree II lesion, and 6 as Degree III lesion. Postoperatively, 34 cases(90%) obtained an anatomical improvement obviously. The percentage of the patients who got excellent and good recovery was above 90%. We conclude that RF-II pedicle screw fixation system has the strongest three-dimensional rigidity and, attains complete and satisfactory decompression. The entire intervertebral disk in the level of the lesion is suggested to be removed. The system is ingeniously and originally designed, and is easy to obtain a simple and an effective reduction of the spondylolisthesis. Additionally, the application of the system can obtain a good interbody fusion by three steps: broadening the interbody space; stuffing the space with broken bones; and compressing the involved vertebral body. The authors considered that the RF-II screw system is one of the most ideal internal fixations for the treatment of lumbar spondylolisthesis.