Objective: We tested the hypothesis that the decline in endothelium-dependent arteriolar dilation in adult rats is reversed by chronic exercise activity.
Methods: Rats were divided into young (8-10 weeks)-sedentary (SED(Y)), adult (29-32 weeks)-sedentary (SED(A)), and adult-exercised (EX(A), treadmill exercise for 18-20 weeks) groups. Responses of isolated arterioles ( approximately 50 microm at 80 mm Hg) of gracilis muscle were assessed to increases in perfusate flow and vasoactive agents.
Results: With no differences in basal tone, maximal flow-induced dilations were not different between arterioles of SED(Y) and SED(A) rats (71 +/- 2 and 72 +/- 2% of passive diameter, respectively), yet the sensitivity of arterioles to shear stress (WSS(50)) was significantly less in SED(A) than in SED(Y) rats (35 +/- 4 vs. 23 +/- 3 dyne/cm(2), respectively). In vessels of EX(A) rats, maximal flow-induced dilation was significantly augmented (88 +/- 2% of passive diameter) and WSS(50) (15 +/- 1 dyn/cm(2)) was significantly reduced. Dilation to acetylcholine was enhanced in arterioles of EX(A), whereas dilation to sodium nitroprusside was not different in vessels of the three groups.
Conclusion: Chronic exercise activity reverses age related reduction in sensitivity of arterioles to increases in wall shear stress.