Abstract
The gene expression patterns of primordial germ cells (PGCs) and embryonic stem cells were analyzed by a modified serial analysis of gene expression. During the process, we cloned a novel gene, PGC7, which was preferentially expressed in PGCs. Immunohistochemical analysis revealed that PGC7 was specifically expressed in early pre-implantation embryos, PGCs and oocytes. These results suggest that PGC7 might play an important role in the development of PGCs and oocytes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Blastocyst / metabolism*
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Blotting, Northern
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Blotting, Western
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Chromosomal Proteins, Non-Histone
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DNA, Complementary / metabolism
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Female
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Gene Expression Regulation, Developmental
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Germ Cells / metabolism*
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Immunohistochemistry
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Male
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Mice
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Molecular Sequence Data
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Oocytes / metabolism
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Protein Biosynthesis*
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Proteins / genetics*
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Time Factors
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Tissue Distribution
Substances
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Chromosomal Proteins, Non-Histone
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DNA, Complementary
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DPPA3 protein, human
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Proteins