Hypercalcemic-type of small cell carcinoma of the ovary: characterization of a new tumor line

Anticancer Res. 2001 Sep-Oct;21(5):3253-9.

Abstract

Background: The aim of this study was to develop and characterize a mouse xenograft model for the hypercalcemic-type of small cell carcinoma of the ovary (HTSCCO).

Patients and methods: Tumor fragments were removed from a patient and cultured in six subsequent generations of nude mice. Histology, comparative genomic hybridization (CGH), electron microscopy and serum calcium levels were investigated.

Results: Morphology remained the same from the primary tumor of the patient through the 6th passage in the mouse. Serum calcium levels were significantly higher in the tumor-bearing mice compared to controls. CGH of the HTSCCO did not show evidence of a close relationship to either a germ cell tumor or an epithelial ovarian cancer.

Conclusion: Some evidence was provided that the HTSCCO is an inhomogeneous tumor that is neither related to a germ cell tumor nor to an epithelial ovarian cancer, but is a distinct tumor entity.

Publication types

  • Case Reports

MeSH terms

  • Adult
  • Animals
  • Calcium / blood
  • Carcinoma, Small Cell / blood
  • Carcinoma, Small Cell / genetics
  • Carcinoma, Small Cell / pathology*
  • Cell Division / physiology
  • Chromosome Aberrations
  • Female
  • Humans
  • Hypercalcemia / blood
  • Hypercalcemia / genetics
  • Hypercalcemia / pathology*
  • Mice
  • Mice, Nude
  • Microscopy, Electron
  • Neoplasm Transplantation
  • Nucleic Acid Hybridization
  • Ovarian Neoplasms / blood
  • Ovarian Neoplasms / genetics
  • Ovarian Neoplasms / pathology*
  • Transplantation, Heterologous
  • Tumor Cells, Cultured*

Substances

  • Calcium