Use of pulsed-field gel electrophoresis, enterobacterial repetitive intergenic consensus typing, and automated ribotyping to assess genomic variability among strains of nontypeable Haemophilus influenzae

M M Pettigrew; B Foxman; Z Ecevit; C F Marrs; J Gilsdorf

doi:10.1128/JCM.40.2.660-662.2002

Use of pulsed-field gel electrophoresis, enterobacterial repetitive intergenic consensus typing, and automated ribotyping to assess genomic variability among strains of nontypeable Haemophilus influenzae

J Clin Microbiol. 2002 Feb;40(2):660-2. doi: 10.1128/JCM.40.2.660-662.2002.

Authors

M M Pettigrew¹, B Foxman, Z Ecevit, C F Marrs, J Gilsdorf

Affiliation

¹ Department of Epidemiology, University of Michigan, Ann Arbor, Michigan 48109-2029, USA.

Abstract

We compared 75 nontypeable (NT) Haemophilus influenzae isolates by pulsed-field gel electrophoresis (PFGE), enterobacterial repetitive intergenic consensus (ERIC)-PCR, and automated ribotyping. PFGE was the most discriminatory of the techniques. ERIC-PCR provides a useful screen but should not replace other techniques as the sole method to group NT H. influenzae strains.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Bacterial Typing Techniques*
Electrophoresis, Gel, Pulsed-Field
Genetic Variation*
Genome, Bacterial*
Haemophilus Infections / microbiology*
Haemophilus influenzae / classification*
Haemophilus influenzae / genetics*
Humans
Polymerase Chain Reaction / methods
Ribotyping
Robotics

Grants and funding

R01-AI25630/AI/NIAID NIH HHS/United States