Highly sensitive assay for human herpesvirus 8 antibodies that uses a multiple antigenic peptide derived from open reading frame K8.1

J Clin Microbiol. 2002 Feb;40(2):325-9. doi: 10.1128/JCM.40.02.325-329.2002.

Abstract

The immunodominant region of the human herpesvirus 8 (HHV-8), the antibody-binding site of glycoprotein K8.1A, was mapped to the N-terminal region by using overlapping peptides and a residue replacement method. The main epitope was located within residues 44 to 56 (GQVYQDWL----C). Based on this information, we developed an enzyme immunoassay to detect HHV-8 antibodies in human sera using a four-branch multiple antigenic peptide as the antigen. The sensitivity and specificity of the assay were 96 and 99.4%, respectively. This assay should be useful for population-based, epidemiological studies of HHV-8 infection.

Publication types

  • Evaluation Study

MeSH terms

  • Amino Acid Sequence
  • Antibodies, Viral / blood*
  • Antigens, Viral / chemistry
  • Antigens, Viral / immunology
  • Epitope Mapping*
  • Glycoproteins / chemistry
  • Glycoproteins / genetics
  • Glycoproteins / immunology*
  • Herpesvirus 8, Human / immunology*
  • Humans
  • Molecular Sequence Data
  • Open Reading Frames / genetics
  • Peptides / chemistry
  • Peptides / immunology*
  • Sarcoma, Kaposi / diagnosis*
  • Sarcoma, Kaposi / virology
  • Sensitivity and Specificity
  • Viral Proteins*

Substances

  • Antibodies, Viral
  • Antigens, Viral
  • Glycoproteins
  • K8.1 protein, Human herpesvirus 8
  • Peptides
  • Viral Proteins