Phase variable restriction-modification systems in Moraxella catarrhalis

FEMS Immunol Med Microbiol. 2002 Jan 14;32(2):159-65. doi: 10.1111/j.1574-695X.2002.tb00548.x.

Abstract

A repetitive DNA motif was used as a marker to identify novel genes in the mucosal pathogen Moraxella catarrhalis. There is a high prevalence of such repetitive motifs in virulence genes that display phase variable expression. Two repeat containing loci were identified using a digoxigenin-labelled 5'-(CAAC)6-3' oligonucleotide probe. The repeats are located in the methylase components of two distinct type III restriction-modification (R-M) systems. We suggest that the phase variable nature of these R-M systems indicates that they have an important role in the biology of M. catarrhalis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA Modification Methylases
  • DNA Restriction Enzymes
  • DNA, Bacterial
  • Humans
  • Microsatellite Repeats
  • Molecular Sequence Data
  • Moraxella catarrhalis / genetics*
  • Moraxella catarrhalis / growth & development
  • Moraxella catarrhalis / physiology
  • Neisseriaceae Infections / microbiology*

Substances

  • DNA, Bacterial
  • DNA Modification Methylases
  • DNA Restriction Enzymes

Associated data

  • GENBANK/AY049056
  • GENBANK/AY049057