CD44 expression in colorectal cancer. An immunohistochemical study including correlation with cathepsin D immunoreactivity and some tumour clinicopathological features

Folia Histochem Cytobiol. 2001:39 Suppl 2:152-3.

Abstract

CD44 is a cell adhesion molecule involved in tumour growth and progression. This study was undertaken to evaluate the expression of CD44 standard protein in a series of 54 colorectal adenocarcinomas in correlation with cathepsin D immunoreactivity and some other clinicopathological variables. Formalin-fixed and paraffin-embedded tissues were investigated with anti-CD44 standard protein and anti-cathepsin D antibody. Immunolocalisation of CD44 protein and cathepsin D was performed using LSAB method. 13 (41.9%) out of 31 carcinomas without lymph-node metastases had positive CD44 expression, whereas only 6 (26.1%) out of 23 carcinomas with lymph-node metastases were found positive for CD44 expression. CD44 expression in carcinomas was positively correlated with tumour cells cathepsin D (p<0.01) immunostaining. statistically significant correlation was found between the expression of CD44 standard protein and the tumour site, age and sex of the patients. These results suggest that the standard-type CD44 protein lymph-node metastases, probably with cooperation of cathepsin D.

MeSH terms

  • Adenocarcinoma / chemistry*
  • Adenocarcinoma / secondary*
  • Aged
  • Cathepsin D / analysis*
  • Colorectal Neoplasms / chemistry*
  • Colorectal Neoplasms / pathology*
  • Female
  • Humans
  • Hyaluronan Receptors / analysis*
  • Immunohistochemistry
  • Lymphatic Metastasis
  • Male
  • Middle Aged
  • Predictive Value of Tests
  • Prognosis

Substances

  • Hyaluronan Receptors
  • Cathepsin D