Biotinylated lithocholic acids for affinity chromatography of mammalian DNA polymerases alpha and beta

Bioorg Med Chem Lett. 2002 Feb 11;12(3):287-90. doi: 10.1016/s0960-894x(01)00725-9.

Abstract

Biotinylated lithocholic acids have been synthesized. The compounds inhibited mammalian DNA polymerases alpha and beta with dose-dependent manner. The streptavidine columns conjugated with the synthetic biotinylated compounds chromatographed both two enzymes eluted by KCl solution at the different concentrations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biotin / chemistry
  • Chromatography, Affinity
  • DNA Polymerase I / antagonists & inhibitors
  • DNA Polymerase I / chemistry*
  • DNA Polymerase I / isolation & purification
  • DNA Polymerase beta / antagonists & inhibitors
  • DNA Polymerase beta / chemistry*
  • DNA Polymerase beta / isolation & purification
  • Enzyme Inhibitors / chemical synthesis
  • Enzyme Inhibitors / chemistry*
  • Enzyme Inhibitors / pharmacology
  • Indicators and Reagents
  • Kinetics
  • Lithocholic Acid / chemical synthesis
  • Lithocholic Acid / chemistry*
  • Lithocholic Acid / pharmacology
  • Magnetic Resonance Spectroscopy
  • Rats
  • Spectrometry, Mass, Fast Atom Bombardment

Substances

  • Enzyme Inhibitors
  • Indicators and Reagents
  • Lithocholic Acid
  • Biotin
  • DNA Polymerase I
  • DNA Polymerase beta