Markers of triglyceride-rich lipoprotein remnant metabolism in visceral obesity

Clin Chem. 2002 Feb;48(2):278-83.

Abstract

Background: Triglyceride-rich lipoprotein remnants are atherogenic, and this may be particularly important in visceral obesity. We investigated remnant metabolism in obese men by measuring remnant-like particle-cholesterol (RLP-C), apolipoprotein (apo) B-48, apoC-III, and the clearance of a labeled remnant-like emulsion.

Methods: Fasting RLP-C, apoB-48, and apoC-III concentrations were measured in 48 viscerally obese men and 10 lean controls. RLP-C was determined by immunoseparation assay, apoB-48 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and enhanced chemiluminescence, and apoC-III by immunoturbidimetric assay. The catabolism of chylomicron remnants was measured by intravenous injection of a remnant-like emulsion containing cholesteryl [(13)C]oleate, with isotopic enrichment of (13)CO(2) in breath determined by isotope-ratio mass spectrometry and a multicompartmental model to estimate fractional catabolic rate (FCR) of the emulsion.

Results: Compared with controls, obese men had significantly increased plasma concentrations of RLP-C, apoB-48, and apoC-III (P <0.001 for all). Plasma total apoB-100, non-HDL-cholesterol, LDL-cholesterol, triglycerides, and insulin resistance (HOMA score) were also significantly higher in the obese group (P <0.001 for all). Obese men had a significantly lower FCR of the remnant-like emulsion compared with controls (P = 0.020).

Conclusions: Viscerally obese individuals have insulin resistance and increased plasma concentrations of triglyceride-rich lipoprotein remnants, which may be attributable to decreased catabolism of these particles.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Apolipoprotein B-48
  • Apolipoprotein C-III
  • Apolipoproteins / blood
  • Apolipoproteins B / blood
  • Apolipoproteins C / blood
  • Biomarkers / blood
  • Cholesterol / blood*
  • Humans
  • Lipoproteins / blood*
  • Male
  • Obesity / blood*
  • Triglycerides / blood*

Substances

  • Apolipoprotein B-48
  • Apolipoprotein C-III
  • Apolipoproteins
  • Apolipoproteins B
  • Apolipoproteins C
  • Biomarkers
  • Lipoproteins
  • Triglycerides
  • remnant-like particle cholesterol
  • Cholesterol